《植物生理学报》 2014, 50(2): 178-184

三个苹果NBS基因的鉴定及其对外源生长物质的响应

柏素花^1,2, 宋霄¹, 张玉刚¹, 戴洪义^1,*

青岛农业大学¹园艺学院, ²生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109

通信作者：戴洪义；E-mail: hydai@qau.edu.cn；Tel: 0532-86080008

摘　要：

为了探讨苹果NBS基因的生理作用, 从苹果中鉴定了NBS家族的3个基因, 分别命名为MdNBS、MdTIR-NBS1和MdTIR-NBS-LRR1。这3个基因编码的蛋白质均含有NB-ARC结构域, MdTIR-NBS1和MdTIR-NBS-LRR1蛋白N端还有TIR结构域, MdTIR-NBS-LRR1蛋白在C端含有LRR结构。荧光定量PCR分析表明, 这3个不同类型的NBS基因的表达具有明显不同的组织特异性, 此外, MdNBS、MdTIR-NBS1和MdTIR-NBS-LRR1在‘嘎啦’苹果幼苗叶片中均受SA和MeJA诱导。ACC处理不能提高MdNBS和MdTIR-NBS1基因的表达, 但能诱导MdTIR-NBS-LRR1基因表达。结果表明, MdNBS、MdTIR-NBS1和MdTIR-NBS-LRR1基因可能参与了苹果抗逆或抗病防御反应。

关键词：苹果; NBS基因; 基因表达; SA; MeJA; ACC

收稿：2013-09-18   修定：2013-12-17

资助：国家苹果产业技术体系项目(CARS-28-01-07)、青岛市科技计划基础研究项目[12-1-4-5-(1)-jch]和“十二五”农村领域国家科技计划项目(2013BAD02B01)。

Identification of Three Apple NBS Genes and Their Expression Responding to Exogenous Phytohormones

BAI Su-Hua^1,2, SONG Xiao¹, ZHANG Yu-Gang¹, DAI Hong-Yi^1,*

¹College of Horticulture, ²Key Lab of Plant Biotechnology of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong 266109, China

Corresponding author: DAI Hong-Yi; E-mail: hydai@qau.edu.cn; Tel: 0532-86080008

Abstract:

To explore the physiological roles of apple NBS genes, three typical NBS genes were identified from Malus×domestica and designated as MdNBS, MdTIR-NBS1 and MdTIR-NBS-LRR1. All the proteins encoded by the three genes contain a NB-ARC domain. In addition, both MdTIR-NBS1 and MdTIR-NBS-LRR1 proteins include a TIR motif in N terminus and MdTIR-NBS-LRR1 protein has a LRR motif in C terminus. The quantitative real-time PCR revealed that the expressions of these three NBS genes varied with different organs. MdNBS was highly expressed in functional leaves, MdTIR-NBS1 in buds and MdTIR-NBS-LRR1 in young leaves, respectively. Expression course analysis demonstrated that the expression of MdNBS, MdTIR-NBS1 and MdTIR-NBS-LRR1 in the young leaves of ‘Gala’ apple could be up-regulated by SA and MeJA. ACC treatment could not enhance the expression of MdNBS and MdTIR-NBS1, but could promote the expression of MdTIRNBS-LRR1. Taken together, the results implied that the three genes might be involved in defense responses against biostress or abiostress in apple.

Key words: Malus×domestica; NBS genes; gene expression; SA; MeJA; ACC